Journal: Oncology Letters
Article Title: Isolation and characterization of adult mammary stem cells from breast cancer-adjacent tissues
doi: 10.3892/ol.2017.6485
Figure Lengend Snippet: AMSCs exhibit differential gene expression compared with autologous breast cancer cells. (A) Representative image of four blocks following the hybridization of Cy3-labeled AMSC sample (green signal) and Cy5-labeled breast cancer sample (red signal). The CapitalBio Human Genome Oligo Array (22K) includes 21,522 70-mer oligo probes spotted on a 75×25-mm, chemically modified glass slide. The green color indicates a stronger signal from the AMSC sample, whereas red indicates a stronger signal from the breast cancer sample, and yellow indicates a similar signal from the two tissue types. (B) Gel electrophoretic analyses of RT-PCR products. Total RNA was extracted from isolated breast cancer cell samples and AMSC samples selected at random from the 9 patients with breast cancer, and was reverse transcribed. The relative gene expression levels of MYC, RAS, and ERBB2 were examined by semi-quantitative PCR, with ACTB used as an internal control. (C) Western blot analysis of Ras, ErbB2 and PTEN in breast cancer cell samples and AMSC samples with β-actin used as internal control. AMSC, adult mammary stem cell; RT-PCR, reverse transcription-polymerase chain reaction; ErbB2, ErbB receptor tyrosine kinase 2; ACTB, β-actin; PTEN, phosphatase and tensin homolog.
Article Snippet: The gene expression profiles of the isolated AMSCs and breast cancer cells of the 9 patients were determined using a CapitalBio Human Genome Oligo Array (22 K) (cat. no. 400010; CapitalBio Corporation, Beijing, China) of 21,552 human genes, according to the manufacturer's instructions.
Techniques: Gene Expression, Hybridization, Labeling, Modification, Reverse Transcription Polymerase Chain Reaction, Isolation, Reverse Transcription, Real-time Polymerase Chain Reaction, Control, Western Blot, Polymerase Chain Reaction
